RNA Processing Part A: General Methods

RNA pseudoknots: Translational frameshifting and readthrough on viral RNAs
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The protein filamin is localized within lamellipodia and filopodia. Filamin is one of the cross-linking proteins of actin. It may play an additional role of linking the cytoskeleton to cell-substratum adhesion Sites Matsudaira, Two independent filamin-like clones were found.

The found associating amino acid sequence of the two filamin clones are shown in FIG. Bellamy, C. Cell death and disease: The biology and regulation of apoptosis. Seminare in Cancer Biology 6, Danen-Van Oorschot, A. M, Den Hollander A.

BAG-1 inhibits pinduced but not apoptin-induced apoptosis, Apoptosis 2, Dreyfuss, G. Annual Review Biochemistry 62, Duke, R. M, Young, J, D-E. Cell suicide in health and disease.


Validation experiments of the genes with the largest splicing changes showed that the alternative exon usage induced by CPT is not observed with cisplatin or vinblastine, which are other two widely used chemotherapeutic agents. The pathobiology of splicing. Please review our Terms and Conditions of Use and check box below to share full-text version of article. The finest audio download rna processing part a general in the book was become by latinorum approaches. Bredenbeek P. Molecular Cloning: A Laboratory Manual. Human mutations in integrator complex subunits link transcriptome integrity to brain development.

Scientific American December , Durfee, T. The retinoblastoma protein associates with the protein phosphate type I catalytic subunit. Genes and Development 7, Barnshaw, W. Nuclear changes in apoptosis.

RNA processing part A : General methods

Current Opinion in Cell Biology 7, Fields, S. A novel genetic system to detect protein-protein interactions. Nature , Gamberi, C. Hockenberry, D. Bcl-2 in cancer development and apoptosis. Journal of Cell Science, Supplement 16, Hoffman, C. A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of Escherichia coil.

Gene 57, Holzmann, K. European Journal Biochemistry , Honore, B. Journal of Biological Chemistry , Kerr, J. Apoptosis: It significance in cancer and cancer therapy. Cancer 73, Klebe, R. Gene 25, Levine, A. Cell 88, Maniatis, T. Molecular Cloning: A Laboratory Manual. Matunis, M. Nucleic Acids Research 22, McDonell T. Implications of apoptotic cell death regulation in cancer therapy. Seminars in Cancer Biology 6, Noteborn, M.

Apoptin induces apoptosis in human transformed and malignant cells but not in normal cells as essential characteristic for the development of an anti-tumor therapy. F,, Van Roozelaar, D. Characterization of cloned chicken anemia virus DNA that contains all elements for the infectious replication cycle. Journal of Virology 65, European Patent Application no. A single chicken anemia virus protein induces apoptosis. Journal of Virology 68, Methods and means for determining the transforming capability of agents, for determining the predisposition of cells to become transformed and prophylactic treatment of cancer using apoptin-like activity.

Rose, M.

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Methods in yeast genetics. A laboratory course manual. Sachs, L. Control of programmed cell death in normal and leukemia cells: New implications for therapy. Blood 82, Sanger, F. DNA sequencing with chain-terminating inhibitors. Telford, W. Comparative evaluation of several DNA binding dyes in the detection of apoptosis-associated chromatin degradation by flow cytometry. Cytometry 13, Teodoro, J. Regulation of apoptosis by viral gene products.

Journal of Virology 71, Thompson, C. B, Apoptosis in the pathogenesis and treatment of disease. Science , The genetic regulation of apoptosis. Current Opinion in Genetics and Development 5, Wyllie, A. Cell death; The significance of apoptosis. International Review of Cytology 68, Yang, X. A protein kinase substrate identified by the two-hybrid system.

Zhuang, S. Apoptin, a protein encoded by chicken anemia virus, induces cell death in various human hematologic malignant cells in vitro. Leukemia 9 S1, Apoptin, a protein derived from chicken anemia virus, induces a pindependent apoptosis in human osteosarcoma cells.

Topic rooms within Cell Biology

Cancer Research 55, Effective date : The invention relates to activation of apoptosis by means of interference with the function of snRNPs and hnRNP-like compounds. Also the invention relates to anti-tumor therapies with compounds, which negatively interfere with snRNPs and hnRNP-like compounds leading to induction of apoptosis, resulting in the elimination of tumor cells. Also the invention relates to therapies for diseases related to aberrant apoptosis induction, such as auto-immune diseases.

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A group of proteins found to be associated with apoptin is the family of hnRNP-like proteins. Bacterial and Yeast Strains The E. Transformants were plated on LB media containing ampicillin. Isolation of Apoptin-associating pACT Clones By means of colony-filter assay the colonies were lysed and hybridized to a radioactive-labeled mer oligomer, which is specific for pACT see also section Sequence analysis.

Post-transcriptional modification

Sequence Analysis The subclones containing the sequence encoding apoptin-associating proteins were sequenced using dideoxy NTP's according to the Sanger method which was performed by Eurogentec, Nederland BV Maastricht, The Netherlands. Results and Discussion Apoptin induces specifically apoptosis in transformed cells, such as cell lines derived from human tumors. Description of Apoptin-associating Proteins The yeast genetic screen for apoptin-associating proteins resulted in the detection of a human homolog of the hnRNP-H. Paulovich, A.

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When checkpoints fail. Steller, H. Mechanisms and genes of cellular suicide. White, E. Life, death, and the pursuit of apoptosis. Genes and development 10, What is claimed is: 1. An expression vector comprising:. An expression vector comprising an isolated nucleic acid molecule encoding an antisense recombinant molecule which is completely complementary to the nucleic acid molecule of SEQ ID NO: 1.

A gene delivery vehicle comprising the expression vector of claim 5. A gene delivery vehicle comprising:. The expression vector of claim 10 , further comprising a nucleic acid sequence encoding apoptin.

mRNA Processing

Research articleFull text access. [4] Synthesis of long, capped transcripts in Vitro by SP6 and T7 RNA polymerases. Joel K. Yisraeli, Doug A. Melton. RNA Processing Part A - 1st Edition - ISBN: , General Methods View all volumes in this series: Methods in Enzymology.

An expression vector comprising an isolated nucleic acid molecule encoding an antisense recombinant molecule which is completely complementary to a nucleic acid molecule encoding the hnRNP protein of SEQ ID NO A gene delivery vehicle comprising the expression vector of claim The gene delivery vehicle of claim 15 , further comprising a nucleic acid sequence encoding apoptin. USB1 en. DEA1 en. A polymerizable composition comprising inorganic particles having a shell of organic. Benzoxazine containing compositions of matter and curable compositions made therewith.

WOA2 en. Ahn et al. Casciola-Rosen, Livia A. Cawthon et al. Harris et al. Hillier et al.